Diagnosis of Genus Helicobacter through a hemi-nested PCR assay of 16S rRNA
نویسندگان
چکیده
منابع مشابه
Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species.
The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
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The genus Lyssavirus is divided into 7 genotypes. Genotype 1 is found worldwide and genotypes 2-4 are found exclusively on the African continent. Representatives of genotypes 1-4 were selected to include every lineage, group and biotype within these genotypes to account for the huge diversity observed in this genus. A reverse transcription PCR as well as a hemi-nested PCR method has been develo...
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In a survey on the presence of archaea in a number of European lakes, it was found that known archaeal primer sets for PCR were not suited for use in freshwater environment, as some lack selectivity, while others were too selective. A nested PCR was developed for denaturing gradient gel electrophoresis (DGGE) with primer sets 21F-958R and Parch519f-Arch915r, respectively. After sequencing of th...
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BACKGROUND Clarithromycin is one of the most important antibiotics for Helicobacter pylori eradication. However, 5-10% of strains are reported to be resistant. It has been shown that one point mutation in the 23S rRNA gene is associated with resistance to clarithromycin. AIMS To establish a polymerase chain reaction (PCR) system which amplifies a segment of the 23S rRNA gene containing the mu...
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ژورنال
عنوان ژورنال: Saudi Pharmaceutical Journal
سال: 2016
ISSN: 1319-0164
DOI: 10.1016/j.jsps.2016.04.015